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To address this, we isolated primary mouse hepatocytes and infected them with adenoviruses expressing Chac1 or GFP, followed by treatment with BSA or BSA-conjugated fatty acids to mimic chow and HFD conditions, respectively

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Or maybe you never had it to begin with because it was just too expensive. I have sat with more patients than I can count and they all say the same thing: losing access is traumatizing, and it permanently changes how people feel about the employers who cut them off